Oligoclonal Antibodies

Oligoclonal antibodies

Clean secondary antibodies with minimal cross-reactivity

Specificity and Sensitivity are the two most important attributes of antibodies used in IHC. For IHC on mouse tissues, due to the similarity between mouse and rat IgGs, it is difficult to make polyclonal antibodies that specifically recognize rat IgG. Non-specific secondary antibodies recognizing mouse IgG (present in most tissues) generate high background. Using monoclonal anti-rat secondary antibody can reduce background significantly, but also decreases signal strength, because only a single epitope is recognized. Our solution is oligoclonal antibodies, which is a mixture of about 10 carefully selected clones of monoclonal antibodies. Oligoclonal antibodies retain the advantages of both monoclonal (specific) and polyclonal (sensitive) antibodies.

Lower background than polyclonal antibodies

There are several situations where both rat and mouse antibodies co-exist. For example, for IHC of mouse-derived tissues, rat antibodies are frequently used. Very likely, mouse antibodies are also present in the target tissues. Another situation is in double staining assays where rat and mouse antibodies are both used. To avoid high background or interference, the anti-rat or anti-mouse secondary antibodies must not cross-react with the co-existing mouse or rat primary antibodies. However, most secondary antibodies on the market are polyclonal antibodies. It is difficult to avoid cross-reactivity without thorough pre-absorbance with mouse or rat immunoglobulins.

Our oligoclonal antibodies do not react with immunoglobulins derived from their own species. Therefore, there is little or no background caused by cross-reactivity when using them as secondary antibodies

Comparison between oligoclonal and polyclonal antibody
A comparison between oligoclonal antibody and polyclonal antibody

Same sensitivities as polyclonal antibodies

Compared to monoclonal antibodies, polyclonal antibodies often have higher sensitivities because they can recognize multiple epitopes on an antigen whereas monoclonal antibodies only recognize one. Our oligoclonal antibodies contain multiple clones of monoclonal antibodies. Therefore, they can recognize several epitopes on the antigen. As a result, they have the same sensitivity as polyclonal antibodies.

Batch consistency

Polyclonal antibodies are prepared from immunized animals. Because immune responses vary greatly between animals, batch variation is unavoidable for polyclonal antibodies. Our oligoclonal antibodies are composed of monoclonal antibodies purified from cell cultures. So batch variation is easily controlled. As a result, you can compare your results from different experiments without worrying about deviations caused by antibody batch variations.

Products available

WZA1069Mouse anti-rat IgG (Fc) oligoclonal antibodiesHRP
WZA1070Mouse anti-rat whole IgG oligoclonal antibodiesHRP

Products coming soon

Mouse anti-rat IgG (Fc) oligoclonal antibodiesFITC
Mouse anti-rat IgG (Fc) oligoclonal antibodiesBiotin
Mouse anti-rat whole IgG oligoclonal antibodiesFITC
Mouse anti-rat whole IgG oligoclonal antibodiesBiotin
Mouse anti-rat IgM oligoclonal antibodiesHRP
Mouse anti-rat IgM oligoclonal antibodiesFITC
Mouse anti-rat IgM oligoclonal antibodiesBiotin
Rat anti-mouse IgG oligoclonal antibodiesHRP
Rat anti-mouse IgG oligoclonal antibodiesFITC
Rat anti-mouse IgG oligoclonal antibodiesBiotin
Mouse F(ab’)2 anti-rat IgG oligoclonal antibodyHRP
Mouse F(ab’)2 anti-rat IgG oligoclonal antibodyAP
Mouse F(ab’)2 anti-rat IgG oligoclonal antibodyFITC
Mouse F(ab’)2 anti-rat IgG oligoclonal antibodyBiotin
Rat F(ab’)2 anti-mouse IgG oligoclonal antibody combinationsHRP
Rat F(ab’)2 anti-mouse IgG oligoclonal antibody combinationsAP
Rat F(ab’)2 anti-mouse IgG oligoclonal antibody combinationsFITC
Rat F(ab’)2 anti-mouse IgG oligoclonal antibody combinationsBiotin