SPEED EvaGreen 2X qPCR Mastermix

SPEED EvaGreen 2X qPCR Mastermix

SKU Description Size Price Shipping  
LYM1013 SPEED EvaGreen 2X qPCR Mastermix with ROX 4 x 1.25 mL $186 Immediate Add To Shopping Cart
LYM1014 SPEED EvaGreen 2X qPCR Mastermix 4 x 1.25 mL $186 Immediate Add To Shopping Cart
Datasheet:
EvaGreen 2X qPCR Mastermix with ROX is designed for quantitative real-time analysis of DNA samples. The components have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in the Mix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.

EvaGreen® is a next-generation DNA-binding dye which similar characteristics to SYBR® Green, but is much brighter and more stable than SYBR® Green. EvaGreen® performance in Real-time PCR surpasses all other similar dyes.

Size   

5 ml (1.25ml x 4 tubes)

Usage   

SNP Genotyping assays
Gene expression analysis
Microarray validation
High throughput screening

Components   

2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, EvaGreen®, ROX, and proprietary buffer components

Storage   

Keep at -20°C. Once thawed, store at 4°C for up to 3 months.

Reaction Setup

EvaGreen 2X Mix:             10 µl
Template DNA:                 < 10ng
Forward Primer:              100-500nM
Reverse Primer:              100-500nM
Water:                               to 20µl


Cycling Program

Activation at 95°C for 10 min
Followed by 40 Cycles with:
   Denature:                95°C 15 sec
   Anneal/Extension:   60°C 1  min

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