SPEED siRNA-IN™, Transfection Reagent for siRNA

SPEED siRNA-IN™, Transfection Reagent for siRNA

SKU Description Size Price Shipping  
LYM1015 SPEED siRNA-IN™, Transfection Reagent for siRNA 1 mL $274 Immediate Add To Shopping Cart
Datasheet:

This reagent is used for efficient transfection of eukaryotic cells with siRNA oligoes.

Size

1 ml

Storage

Keep at 4°C. Do not freeze.

Protocol

These conditions are recommended as guidelines only. You may need to do optimization based on your special needs. A 6-well or 35 mm dish is adequate for most applications, but larger vessels are sometimes required. In that case, consult table 1 in the attached datasheet.

  1. In a 6-well plate, seed cells at a density of 1-3x105 per well in 2.0ml of the appropriate growth medium (with serum if cells are cultured in presence of serum). Incubate the cells at 37°C until cells are 60-90% confluent. This will usually take 18-24 hours, depending on cell types. Since transfection efficiency is sensitive to confluence, it is important to maintain a standard seeding protocol from experiment to experiment.
  2. Prepare the following solutions in sterile 12x75mm or microcentrifuge tubes:
    Solution A: For each transfection, dilute 1-3µg of RNAi oligoes into 125µl serum-free medium.
    Solution B: For each transfection, dilute 4-10µl of SPEED siRNA-IN™ Transfection Reagent into 125µl of serum-free medium. (Vortex SPEED siRNA-IN™ Transfection Reagent before use)
  3. Combine the solutions, mix thoroughly and incubate at room temperature for 20 mins to allow DNA-liposome complexes to form.
  4. Remove growth medium from the cells and add 800µl of serum-free medium to the cells.
  5. Add the Transfection Reagent-Oligo complexes to the cells, mix gently to ensure uniform distribution and incubate at 37°C for 2-24 hours. We recommend starting with 5 hours.
  6. Remove the transfection solution and add 2.0ml of appropriate complete growth medium (with serum) to each well. Incubate cells at 37°C for 24-72 hours before assay.
Other Image