Enterokinase

Enterokinase

SKU Description Size Price Shipping  
LYP1024 Enterokinase 100 U $200 2-5 Days Add To Shopping Cart
Datasheet:
The enterokinase included in this kit is the catalytic subunit of the native holoenzyme, and is highly active and specific for cleaving fusion proteins with the recognition sequence, DDDDK, in the interdomain linker.

Because this product is produced from mammalian expression system, it is highly glycosylated and shows extremely specific cleavage activity compared to other E. coli produced enterokinases. The purified enterokinase is shown as a 47kD band under denaturing and reducing conditions on SDS-PAGE.

Size   

100U/50µL

Components   

1.    Enterokinase: 100 units in 50 µl (1 U of enterokinase cleaves 50 µg of Cleavage Control Protein to 95% completion in 16 hr at 25°C)
2.    Cleavage Control Protein 50 µg in 25 µl
3.    10 X Reaction Buffer (20mM CaCl2; 500mM NaCl; 200mM Tris-HCl pH8.0)

Storage   

Store at -20°C. Stable for at least 3 months.
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