Home/ Products / Full-length Membrane Proteins
DMF1011-50ug
50 ug
$1,800
DMF1011-100ug
100 ug
$2,400
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form were produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles.
This specific membrane protein, the human full length CLDN6 protein (Uniprot ID: P56747), has a MW of 23 kDa.
| gene name | CLDN6 |
| alias | Claudin 6, Claudin-6, Skullin,Claudin6 |
| gene id | Uniprot ID: P56747 |
| host species | Human |
| production host | HEK293 |
| formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose was added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
Elisa plates were pre-coated with 0.5ug/well purified human CLDN6 full length membrane nanoparticles. Serial diluted anti-CLDN6 monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-CLDN6 monoclonal antibody binding with CLDN6 full length membrane nanoparticles is 34.36ng/ml.
FACS analysis of CLDN6 MNPs A. Negative Control 1: CLDN6 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CLDN6 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CLDN6 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CLDN6 full length membrane nanoparticles samples were stained with anti-CLDN6 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody.
DMF1011-50ug
50 ug
$1,800
DMF1011-100ug
100 ug
$2,400