Human ADORA2A Full Length Transmembrane Protein (MNP, Membrane Nanoparticle)

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PRODUCT

  • SKU

    DMF1014-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1014-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form were produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles.
This specific membrane protein, the human full length ADORA2A protein (Uniprot ID: P29274), has a MW of 44.7 kDa.
gene name ADORA2A
alias A2aR, ADORA2, RDC8
gene id Uniprot ID: P29274
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose was added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with 0.5ug/well purified human ADORA2A full length membrane nanoparticles. Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-Flag monoclonal antibody binding with ADORA2A full length membrane nanoparticles is 6.796ng/ml.

FACS analysis of ADORA2A MNPs A. Negative Control 1: ADORA2A full length membrane nanoparticles samples were stained only with Goat anti-mouse lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-ADORA2A antibody (R&D systems, MAB9497R) at 2ug/mL, followed by Goat anti-mouse IgG 488 secondary antibody. C. Negative Control 3: ADORA2A full length membrane nanoparticles samples were stained with anti-His antibody (an irrelevant antibody) at 2ug/mL, followed by Goat anti-mouse IgG 488 secondary antibody. D. ADORA2A full length membrane nanoparticles samples were stained with anti-ADORA2A antibody (R&D systems, MAB9497R) at 2ug/mL, followed by Goat anti-mouse IgG 488 secondary antibody.

Related Products

PRODUCT

  • SKU

    DMF1014-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1014-100ug

  • Size

    100 ug

  • Price

    $2,400

1

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