Human GPRC5D Full Length Transmembrane Protein (Synthetic Nanodisc) with Flag Tag

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PRODUCT

  • SKU

    DMF1025-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1025-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1025-100ug

  • Size

    100 ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length GPRC5D Protein has a MW of 38.6 kDa.
target GPRC5D
gene name GPRC5D
alias GPRC5D
gene id Uniprot ID: Q9NZD1
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were added with Flag Tag GPRC5D-Nanodisc (0.5 ug per well) on an anti-Flag monoclonal antibody pre-coated (0.5 ug per well) plate. Serial diluted anti-GPRC5D monoclonal antibodysolutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPRC5D monoclonal antibody binding with GPRC5D-Nanodisc is 32.86 ng/ml.

Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE


PRODUCT

  • SKU

    DMF1025-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1025-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1025-100ug

  • Size

    100 ug

  • Price

    $12,000

1