Human GRM7 Full Length Transmembrane Protein (Synthetic Nanodisc)

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PRODUCT

  • SKU

    DMF1124-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1124-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1124-100ug

  • Size

    100 ug

  • Price

    $12,000

1
Synthetic nanodiscs were produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs were further purified, offering intact membrane proteins in their natural configuration.
This specific membrane protein, the human full length GRM7 protein (Uniprot ID: Q14831), has a MW of 102.3 kDa.
gene name GRM7
alias GLUR7, GPRC1G, MGLU7, MGLUR7, NEDSHBA, PPP1R87
gene id Uniprot ID: Q14831
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose was added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with Flag Tag GRM7-Nanodisc (0.2ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-Flag monoclonal antibody binding with GRM7-Nanodisc is 2.478ng/ml.

Human GRM7-Nanodisc, Flag Tag on SDS-PAGE

PRODUCT

  • SKU

    DMF1124-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1124-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1124-100ug

  • Size

    100 ug

  • Price

    $12,000

1

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