Human BTN3A1 (Glu30-Gly254) protein, with C-mFc and 6xHis Tag, expressed from HEK293

PRODUCT

This recombinant protein contains Glu30-Gly254 of Uniprot ID O00481. It has a C-mFc and 6xHis Tag, with a calculated molecular weight of 51.36 kDa.

gene name	BTN3A1
gene id	Uniprot ID: O00481
alias	BTN3A1, BTF5, CD277, BTN3.1, BT3.1
host species	Human
production host	HEK293
tags	C-mFc and 6xHis Tag
purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation	Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions.
storage	Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

ELISA plate coated with 1 ug/mL (100 uL/well) Human BTN3A1 protein, mFc-His Tag binds to anti-BTN3A1 Rabbit mAb in a linear range of 3.2-16 ng/mL.

Human BTN3A1 Protein, mFc-His Tag on SDS-PAGE under non-reducing (NR) and reducing (R) conditions.