Human CD80/B7-1 (Val35-Asn242) protein, with C-mFc and 6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1313-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1313-100ug

  • Size

    100 ug

  • Price

    $971

1
This recombinant protein contains Val35-Asn242 of Uniprot ID P33681. It has a C-mFc and 6xHis Tag, with a calculated molecular weight of 50.9 kDa. In SDS-PAGE, the protein migrates at 70-130 kDa due to glycosylation.
gene name CD80
gene id Uniprot ID: P33681
alias CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
host species Human
production host HEK293
tags C-mFc and 6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 1 ug/ml (100 ul/well) Human B7-1, mFc-His tagged protein binds to anti-B7-1 monoclonal antibody,Rabbit mAb clone: DM111 in a linear range of 0.13-80 ng/ml.

Flow cytometry analysis of 15 ug/mL Human B7-1 Protein, mFc-His tag on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human B7-1 Protein, mFc-His Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1313-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1313-100ug

  • Size

    100 ug

  • Price

    $971

1