Human IL2 Superkine mutant protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1734-50ug

  • Size

    50 ug

  • Price

    $299

1

This recombinant protein contains Ala21-Thr153 of Uniprot ID P60568, with 5 mutations: L100F, R101D, L105V, I106V and I112F). It has a C-6xHis Tag, with a molecular weight of 16.5 KDa.

gene name IL2
gene id Uniprot ID: P60568
alias Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2
host species Human
production host HEK293
tags C-6xHis Tag
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from a 0.2 um filtered solution of 4mM HCl.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Loaded Human IL-2RB-Fc on Protein A Biosensor, binds to Human IL-2 Superkine with an affinity constant of 0.58 nM as determined in BLI assay.

Measured in a cell proliferation assay using CTLL?2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.

Loaded Human IL-2RA-Fc on Protein A Biosensor, binds to Human IL-2 Superkine with an affinity constant of 10.4 nM as determined in BLI assay.

Purity greater than 95% as determined by reducing SDS-PAGE.


PRODUCT

  • SKU

    DMP1734-50ug

  • Size

    50 ug

  • Price

    $299

1