Human IL4/IL-4 protein, with C-Fc Tag, expressed from HEK293

PRODUCT

This recombinant protein contains the amino acid sequence of Uniprot ID P05112. It has a C-Fc Tag, with a molecular weight of 41.9 KDa.

gene name	IL4
gene id	Uniprot ID: P05112
alias	Interleukin-4, IL-4, B-Cell Stimulatory Factor 1, BSF-1, Binetrakin, Lymphocyte Stimulatory Factor 1, Pitrakinra, IL4
host species	Human
production host	HEK293
tags	C-Fc Tag
purity	Greater than 95% as determined by reducing SDS-PAGE.

endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation	Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions.
storage	Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Loaded Cynomolgus IL-4RA-His on HIS1K Biosensor, binds to Human IL-4-Fc with an affinity constant of <10-3 nM as determined in BLI assay.

Loaded Human IL-4RA-His on HIS1K Biosensor, binds to Human IL-4-Fc with an affinity constant of <10-3 nM as determined in BLI assay.

Immobilized Human IL-4-Fc at 5ug/ml (100 ul/well) binds to Cynomolgus IL-4Ra-His. The ED50 of Recombinant Human IL-4Ra-His is 79.49 ng/ml.

Purity greater than 95% as determined by reducing SDS-PAGE.