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DMP1777-50ug
50 ug
$648
DMP1777-100ug
100 ug
$971
gene name | IL6 |
gene id | Uniprot ID: P05231 |
alias | IL6, Interleukin-6, BSF2, HSF, IFNB2 |
host species | Human |
production host | HEK293 |
tags | C-mFc and 6xHis Tag |
purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
endotoxin | No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug). |
formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization. |
reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
shipping | Shipped at ambient conditions. |
storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
ELISA plate coated with 2 ug/ml (100 ul/well) Human IL6, mFc-His tagged protein binds to anti-IL6 neutralizing antibody in a linear range of 7.81-386.8 ng/ml.
ELISA plate coated with 2 ug/ml (100 ul/well) Human IL6R, His tagged protein binds to Human IL6, mFc-His tagged protein in a linear range of 0.512-40000 ng/ml.
ELISA plate coated with 1 ug/ml (100 ul/well) Human IL6R, hFc tagged protein binds to Human IL6, mFc-His tagged protein in a linear range of 14.4-360 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human IL6R (A) were surface stained with Human IL6, mFc-His tagged protein 1ug/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Human IL6 Protein, mFc-His Tag, SDS-PAGE analysis under reducing conditions.
DMP1777-50ug
50 ug
$648
DMP1777-100ug
100 ug
$971