Canine PDCD1/PD-1 (Leu25-Gly168) protein, with C-hFc tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1970-50ug

  • Size

    50 ug

  • Price

    $751

1
  • SKU

    DMP1970-100ug

  • Size

    100 ug

  • Price

    $1,129

1
This recombinant protein contains Leu25-Gly168 of Uniprot ID A0A090BAM7. It has a C-hFc tag, with a calculated molecular weight of 42.3 kDa. In SDS-PAGE, the protein migrates at 55-70 kDa due to glycosylation.
gene name PDCD1
gene id Uniprot ID A0A090BAM7
alias CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
host species Canine
production host HEK293
tags C-hFc tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag binds to anti-Canine PD-1 (INTERVET 4F12) mAb in a linear range of 0.64?80 ng/mL.

ELISA plate coated with 2 ug/mL (100 uL/well) Canine PD-1 Protein, hFc Tag binds to Canine PD-L1 Protein, mFc Tag in a linear range of 16?400 ng/mL.

Flow cytometry analysis of 1ug/mL Canine PD-1 Protein, hFc Tag on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Canine PD-1 Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1970-50ug

  • Size

    50 ug

  • Price

    $751

1
  • SKU

    DMP1970-100ug

  • Size

    100 ug

  • Price

    $1,129

1