Recombinant rabbit anti-human CD244/2B4 monoclonal antibody, clone DM70

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PRODUCT

  • SKU

    DMR1058-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1058-500ug

  • Size

    500 ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CD244
alias CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
gene id Uniprot ID: Q9BZW8
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM70
iso type Rabbit IgG
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Rabbit anti-2B4 monoclonal antibody 1ug/ml (clone: DM70) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

ELISA plate coated with 2 ug/ml (100 ul/well) Human 2B4 protein, mFc-His tagged protein binds to Rabbit anti-2B4 monoclonal antibody (clone: DM70) in a linear range of 1-100 ng/ml.

Flow cytometry data of serially titrated Rabbit anti-2B4 monoclonal antibody (clone: DM70) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-2B4 mAb clones by titration of different concentration onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Related Products

PRODUCT

  • SKU

    DMR1058-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1058-500ug

  • Size

    500 ug

  • Price

    $2,010

1

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