Recombinant rabbit anti-His Tag monoclonal antibody, clone DM1

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PRODUCT

  • SKU

    DMR1288-100ug

  • Size

    100 ug

  • Price

    $599

1
  • SKU

    DMR1288-500ug

  • Size

    500 ug

  • Price

    $1,799

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target His Tag
host species Rabbit
production host HEK293
reactivity Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus.
clonality Monoclonal
clone number DM1
iso type Rabbit IgG
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, WB
dilution WB 1:1000
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Western blot analysis of his-tag fusion protein using His-Tag antibody at 1:1000 dilution. Secondary antibody: HRP Goat anti-Rabbit IgG (H L) at 1:5000 dilution. His-tag fusion protein : 70kDa, 20ng per lane. Blocking buffer: 5% nonfat dry milk in TBST. Exposure time: 1s.

PRODUCT

  • SKU

    DMR1288-100ug

  • Size

    100 ug

  • Price

    $599

1
  • SKU

    DMR1288-500ug

  • Size

    500 ug

  • Price

    $1,799

1

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