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DMS1063-100ug
100 ug
$350
target | EGFR |
alias | EGFR, ERBB, ERBB1, HER1, PIG61, mENA |
gene id | Uniprote ID: P00533 |
host species | Chimeric |
reactivity | Human |
iso type | IgG1 |
formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5% - 8% trehalose is added as a protective agent prior to lyophilization. |
reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 minutes with occasional gentle tapping. It is recommended that 0.1% HSA be added as a carrier protein for long-term storage. |
applications | ELISA; Flow Cytometry |
dilution | ELISA 1:5000-10000 |
shipping | Shipped at ambient conditions. |
storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
note | Other commonly used names in publications refering to this antibody: Fab C225, IMC-225 |
ELISA plate coated with 2 ug/ml (100 ul/well) Human EGFR, His tagged protein binds to anti-EGFR neutralizing antibody in a linear range of 0.64-80 ng/ml.
EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis of anti-EGFR (Cetuximab) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)
DMS1063-100ug
100 ug
$350