Biotinylated human CEACAM5 (Lys35-Ala685) protein, with C-10xHis-Avi Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1018-25ug

  • Size

    25ug

  • Price

    $552

1
  • SKU

    YCD1018-200ug

  • Size

    200ug

  • Price

    $2,394

1
This recombinant protein contains Lys35-Ala685 of UniProt P06731. It has a His tag at the C-terminus, followed by an Avi tag. The protein's calculated molecular weight is 75.0 kDa, migrating at 90-120 kDa in reducing SDS-PAGE due to glycosylation.
gene name CEACAM5
gene id UniProt P06731
host species Human
production host HEK293
tags C-10xHis-Avi
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 20 mM Tris, 150 mM Potassium glutamate, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human CEACAM-5 protein

ELISA: Immobilized Biotinylated Human CEACAM-5-His-Avi at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate. binds to Anti-CEACAM-5 (labetuzumab) Antibody with a linear range of 0.3-10 ng/mL.

ELISA: Immobilized Anti-CEACAM-5 (labetuzumab) Antibody at 1 ug/mL (100 uL/well) binds to Biotinylated Human CEACAM-5-His-Avi with a linear range of 0.6-20 ng/mL .


PRODUCT

  • SKU

    YCD1018-25ug

  • Size

    25ug

  • Price

    $552

1
  • SKU

    YCD1018-200ug

  • Size

    200ug

  • Price

    $2,394

1