Human VCAM1 / VCAM-1 (Phe25-Glu698) protein, with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1044-100ug

  • Size

    100ug

  • Price

    $375

1
  • SKU

    YCD1044-1mg

  • Size

    1mg

  • Price

    $2,475

1
This recombinant protein contains Phe25-Glu698 of UniProt P19320. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 100.7 kDa, migrating at 126 kDa+/-5 kDa in reducing SDS-PAGE due to glycosylation.
gene name VCAM1
gene id UniProt P19320
host species Human
production host HEK293
tags C-Fc
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human VCAM-1 protein

ELISA: Immobilized Human VCAM-1 Protein-Fc at 5 ug/mL (100 uL/well) binds to Human ITGA4&ITGB1 Heterodimer Protein-His&Tag Free with a linear range of 0.039-0.625 ug/mL.

ELISA: Immobilized Biotinylated Human ITGA4&ITGB1 Heterodimer Protein-His-Avi&Tag Free at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate binds to Human VCAM-1 Protein-Fc with a linear range of 0.049-0.781 ng/mL .


PRODUCT

  • SKU

    YCD1044-100ug

  • Size

    100ug

  • Price

    $375

1
  • SKU

    YCD1044-1mg

  • Size

    1mg

  • Price

    $2,475

1