Human TNFSF4 / OX40 Ligand (Gln51-Leu183) protein, with N-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1189-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCD1189-1mg

  • Size

    1mg

  • Price

    $3,270

1
This recombinant protein contains Gln51-Leu183 of UniProt P23510. It has a human IgG1 Fc tag at the N-terminus. The protein's calculated molecular weight is 74.0 kDa, migrating at 140-200 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
gene name TNFSF4
gene id UniProt P23510
host species Human
production host HEK293
tags N-Fc
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human OX40 Ligand protein

ELISA: Immobilized Human OX40 Protein-His at 2 ug/mL (100 uL/well) binds to Human OX40 Ligand-Fc with a linear range of 0.1-1 ng/mL.

ELISA: Immobilized Cynomolgus / Rhesus macaque OX40-His at 2 ug/mL (100 uL/well) binds to Human OX40 Ligand-Fc with a linear range of 0.2-3 ng/mL .


PRODUCT

  • SKU

    YCD1189-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCD1189-1mg

  • Size

    1mg

  • Price

    $3,270

1