Human HGF (Gln32-Ser728) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1205-20ug

  • Size

    20ug

  • Price

    $225

1
  • SKU

    YCD1205-100ug

  • Size

    100ug

  • Price

    $570

1
  • SKU

    YCD1205-1mg

  • Size

    1mg

  • Price

    $2,700

1
This recombinant protein contains Gln32-Ser728 of UniProt P14210. It has a His tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. The protein's calculated molecular weight is 81.6 kDa (alpha chain 53.7 kDa and beta chain 27.9 kDa), migrating at 80-90 kDa (alpha and Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) in reducing SDS-PAGE due to glycosylation.
gene name HGF
host species Human
production host HEK293
tags C-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human HGF protein

ELISA: Immobilized Human HGF-His at 2 ug/mL (100 uL/well) binds to Human HGF R-Fc with a linear range of 1-39 ng/mL.


PRODUCT

  • SKU

    YCD1205-20ug

  • Size

    20ug

  • Price

    $225

1
  • SKU

    YCD1205-100ug

  • Size

    100ug

  • Price

    $570

1
  • SKU

    YCD1205-1mg

  • Size

    1mg

  • Price

    $2,700

1