Human PLAU /uPA (Ser21-Leu431) protein, enzyme activated by trypsin, with C-6xHis tag, expressed from HEK293

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  • SKU

    YCD1330-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCD1330-1mg

  • Size

    1mg

  • Price

    $3,960

1
This recombinant protein contains Ser21-Leu431 of UniProt P00749. It has a His tag at the C-terminus. The active form of PLAU is a disulfide-linked heterodimer composed of long chain A (Ser21-Phe177) and chain B (Ile179-Leu431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys156-Phe177) and N-terminal fragment (Ser21-Lys155) with calculated MW of 15.3 kDa, migrating at 17 kDa (N-terminal fragment), 32-35 kDa (chain B) and 45-50 kDa (long chain A & chain B) under non-reducing conditions in SDS-PAGE due to glycosylation.
gene name PLAU
gene id UniProt P00749
host species Human
production host HEK293
tags C-6xHis
purity > 90% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of 25 mM HEPES, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human PLAU protein


PRODUCT

  • SKU

    YCD1330-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCD1330-1mg

  • Size

    1mg

  • Price

    $3,960

1