Cynomolgus TGFB1 / LAP (TGF-beta 1) (Leu30-Ser390, C33S) protein, with N-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1338-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCD1338-1mg

  • Size

    1mg

  • Price

    $4,290

1
This recombinant protein contains Leu30-Ser390(C33S) of UniProt A0A2K5TJB2 (C33S). It has a His tag at the N-terminus. The precursor proprotein is partially cleaved by FURIN to form mature Transforming growth factor beta-1 (TGF-beta-1) and Latency-associated peptide (LAP) chains with calculated MW of 12.7 kDa and 30.4 kDa respectively, migrating at 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) in reducing SDS-PAGE due to glycosylation.
gene name TGFB1
host species Cynomolgus
production host HEK293
tags N-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Cynomolgus Latent TGF-Beta 1 (C33S) protein

ELISA: Immobilized Cynomolgus Latent TGF-Beta 1 (C33S)-His at 2 ug/mL (100 uL/well) binds to Biotinylated Human ITGAV&ITGB6 Heterodimer Protein-His-Avi&Tag Free with a linear range of 1-31 ng/mL.

ELISA: Immobilized Cynomolgus Latent TGF-Beta 1 (C33S)-His at 2 ug/mL (100 uL/well) binds to Biotinylated Human ITGAV&ITGB8 Heterodimer Protein-His-Avi&Tag Free with a linear range of 5-78 ng/mL .

Related Products

PRODUCT

  • SKU

    YCD1338-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCD1338-1mg

  • Size

    1mg

  • Price

    $4,290

1

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