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YCD1586-100ug
100ug
$525
YCD1586-1mg
1mg
$3,465
This recombinant protein contains Ala22-Arg788 of UniProt P01130. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 111.2 kDa. As a result of glycosylation, the protein migrates as 120-125 kDa and 140-150 kDa under reducing (R) condition, and 270-330 kDa under non-reducing (NR) condition (SDS-PAGE).
| gene name | LDLR |
| gene id | UniProt P01130 |
| host species | Human |
| production host | HEK293 |
| tags | C-Fc |
| purity | > 95% as determined by SDS-PAGE |
| endotoxin | No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug). |
| formulation | Protein was lyophilized from a solution in 50 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5, with trehalose added as protectant. |
| reconstitution | Reconstitute lyophilized powder with 250 ul of water to a concentration of 400 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| shipping | Shipped at ambient conditions |
| storage | Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE analysis of Human LDL R protein
ELISA: Immobilized Human PCSK9 (D374Y)-His at 5 ug/mL (100 uL/well) binds to Human LDL R-Fc with a linear range of 0.005-1.25 ug/mL.
ELISA: Immobilized Human LDL R-Fc at 5 ug/mL (100 uL/well) binds to Human PCSK9 (D374Y)-His with a linear range of 10-78 ng/mL .
YCD1586-100ug
100ug
$525
YCD1586-1mg
1mg
$3,465