Human KRAS (Thr2-Cys185) protein, active enzyme, with N-10xHis tag, expressed from E. coli

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PRODUCT

  • SKU

    YCD1621-100ug

  • Size

    100ug

  • Price

    $600

1
  • SKU

    YCD1621-1mg

  • Size

    1mg

  • Price

    $4,200

1
This recombinant protein contains Thr2-Cys185of UniProt P01116. It has a His tag at the N-terminus. The protein's calculated molecular weight is 23.0 kDa, migrating at 25-27 kDa under reducing conditions in SDS-PAGE.
gene name KRAS
gene id UniProt P01116
host species Human
production host E. coli
tags N-10xHis
purity > 95% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of 20 mM Tris,500 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human KRAS protein


PRODUCT

  • SKU

    YCD1621-100ug

  • Size

    100ug

  • Price

    $600

1
  • SKU

    YCD1621-1mg

  • Size

    1mg

  • Price

    $4,200

1