Human ERBB2 (Her2) and ERBB3 (Her3) heterodimer protein, with C-Fc, expressed from HEK293

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PRODUCT

  • SKU

    YCD1661-100ug

  • Size

    100ug

  • Price

    $600

1
  • SKU

    YCD1661-1mg

  • Size

    1mg

  • Price

    $3,900

1
This recombinant heterodimer protein contains Thr23-Thr652 of UniProt P04626 and Ser20-Thr643 of UniProt P21860. Each chain has a calculated MW of 95.9 kDa and 94.9 kDa, migrating at 110-130 kDa in reducing SDS-PAGE due to glycosylation.
gene name ERBB2, ERBB3
gene id UniProt P04626, P21860
host species Human
production host HEK293
tags C-Fc & C-Fc
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Her2 & ErbB3 protein

ELISA: Immobilized Human Her2 & ErbB3 Protein-Fc&Fc Tag at 1 ug/mL (100 uL/well) binds to Trastuzumab with a linear range of 0.2-4 ng/mL.

ELISA: Immobilized Human Her2 & ErbB3 Protein-Fc&Fc Tag at 1 ug/mL (100 uL/well) binds to Monoclonal Anti-HER3 Antibody, Mouse IgG2a with a linear range of 0.1-4 ng/mL .


PRODUCT

  • SKU

    YCD1661-100ug

  • Size

    100ug

  • Price

    $600

1
  • SKU

    YCD1661-1mg

  • Size

    1mg

  • Price

    $3,900

1