Rat ICOSLG / B7-H2 (Glu25-Lys261) protein, with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1678-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCD1678-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains Glu25-Lys261 of NCBI Accession# XP_006256322.1. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 53.4 kDa, migrating at 70-100 kDa in reducing SDS-PAGE due to glycosylation.
gene name ICOSLG
gene id NCBI Accession# XP_006256322.1
host species Rat
production host HEK293
tags C-Fc
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Rat B7-H2 protein

ELISA: Immobilized Mouse ICOS (C137S, C138S)-His at 5 ug/mL (100 uL/well) binds to Rat B7-H2-Fc with a linear range of 0.6-39 ng/mL.

ELISA: Immobilized Biotinylated Human ICOS (C136S, C137S)-His-Avi (recommended for biopanning) at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate binds to Rat B7-H2-Fc with a linear range of 2-39 ng/mL .


PRODUCT

  • SKU

    YCD1678-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCD1678-1mg

  • Size

    1mg

  • Price

    $2,970

1