Dengue virus 2 (strain Thailand/16681/1984) envelope protein, with N-10xHis tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1760-50ug

  • Size

    50ug

  • Price

    $675

1
  • SKU

    YCD1760-500ug

  • Size

    500ug

  • Price

    $4,200

1
This recombinant protein contains Met281-Ser677 of UniProt P29990. It has an N-10xHis tag and was expressed from HEK293. The protein's calculated molecular weight is 46.2 kDa, but migrates at 55-60 kDa under reducing conditions in SDS-PAGE due to glycosylation.
host species Dengue virus
production host HEK293
tags N-10xHis
purity >90% as determined by SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation The protein was lyophilized from a solution of PBS, pH7.4, 0.5M Arginine, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of DENV2 (strain Thailand/16681/1984) Envelope protein

ELISA: Immobilized DENV2 (strain Thailand/16681/1984) Envelope Protein-His at 1 ug/mL (100 uL/well) binds to Human Anti-E(DENV) Antibody EDE2 A11,Human IgG1 | Human Kappa with a linear range of 1-39 ng/mL.


PRODUCT

  • SKU

    YCD1760-50ug

  • Size

    50ug

  • Price

    $675

1
  • SKU

    YCD1760-500ug

  • Size

    500ug

  • Price

    $4,200

1