Biotinylated human KLRK1 / NKG2D (Ile73-Val216) protein, with N-Avi & N-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1920-25ug

  • Size

    25ug

  • Price

    $552

1
  • SKU

    YCD1920-200ug

  • Size

    200ug

  • Price

    $2,173.5

1
This recombinant protein contains Ile73-Val216 of UniProt P26718. It has an Avi tag at the N-terminus, followed by a His tag. The protein's calculated molecular weight is 20.2 kDa, migrating at 28-43 kDa in reducing SDS-PAGE due to glycosylation.
gene name KLRK1
gene id UniProt P26718
host species Human
production host HEK293
tags N-Avi & N-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human NKG2D protein

ELISA: Immobilized Human MICA-Fc at 5 ug/mL (100 uL/well) binds to Biotinylated Human NKG2D Protein-Avi-His with a linear range of 5-156 ng/mL.

ELISA: Immobilized Biotinylated Human NKG2D Protein-Avi-His at 1 ug/mL (100 uL/well) on streptavidin (STN-N5116) precoated (0.5 ug/well) plate binds to Human MICA-Fc with a linear range of 0.05-0.8 ng/mL .


PRODUCT

  • SKU

    YCD1920-25ug

  • Size

    25ug

  • Price

    $552

1
  • SKU

    YCD1920-200ug

  • Size

    200ug

  • Price

    $2,173.5

1