Human SIGLEC10 / Siglec-10 (Met17-Thr546) protein, with C-Fc(A3) Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD1985-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1985-1mg

  • Size

    1mg

  • Price

    $3,810

1
This recombinant protein contains Met17-Thr546 of UniProt Q96LC7. It has a human IgG1 Fc (L118A, G120A, E201A) tag at the C-terminus. The protein's calculated molecular weight is 84.6 kDa. As the result of the mutations for Fc-Tag, the protein migrates as 90-110 kDa (Siglect-10 with Fc-Tag), 60-67 kDa (Siglect-10) and 30-32 kDa (Fc fragment) in reducing SDS-PAGE due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcgammaRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
gene name SIGLEC10
gene id UniProt Q96LC7
host species Human
production host HEK293
tags C-Fc(A3)
purity > 85% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Siglec-10 protein

ELISA: Immobilized Human Siglec-10, Fc (L118A, G120A, E201A) Tag at 5 ug/mL (100 uL/well) binds to Anti-Siglec-10 Antibody with a linear range of 0.2-6 ng/mL.


PRODUCT

  • SKU

    YCD1985-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCD1985-1mg

  • Size

    1mg

  • Price

    $3,810

1