Biotinylated human HAVCR2 / TIM-3 (Ser22-Arg200) protein, with C-Fc-Avi Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCD2009-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD2009-200ug

  • Size

    200ug

  • Price

    $2,070

1
This recombinant protein contains Ser22-Arg200 of UniProt Q8TDQ0. It has a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein's calculated molecular weight is 48.0 kDa, migrating at 65-70 kDa in reducing SDS-PAGE due to glycosylation.
gene name HAVCR2
gene id UniProt Q8TDQ0
host species Human
production host HEK293
tags C-Fc-Avi
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Human TIM-3 protein

ELISA: Immobilized Biotinylated Human TIM-3 Protein-Fc-Avi at 1 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate binds to Monoclonal Anti-Human TIM-3 Antibody4 with a linear range of 0.1-2 ng/mL.


PRODUCT

  • SKU

    YCD2009-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD2009-200ug

  • Size

    200ug

  • Price

    $2,070

1