Human GPC3 / Glypican 3 (Gln25-His559, S509A) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5427-100ug

  • Size

    100ug

  • Price

    $480

1
  • SKU

    YCP5427-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains Gln25-His559 of UniProt P51654 (S359F). It has a His tag at the C-terminus. The protein's calculated molecular weight is 61.7 kDa, migrating at 66-115 kDa in reducing SDS-PAGE due to glycosylation.
gene name GPC3
gene id UniProt P51654 (S359F)
host species Human
production host HEK293
tags C-6xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Glypican 3 (S359F) protein

ELISA: Immobilized Human Glypican 3 (S359F) Protein-His at 0.5 ug/mL (100 uL/well) binds to Monoclonal Anti-Human GPC3 Antibody with a linear range of 0.1-2 ng/mL.

ELISA: Immobilized Human Glypican 3 (S359F) Protein-His at 0.5 ug/mL (100 uL/well) binds to Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL .


PRODUCT

  • SKU

    YCP5427-100ug

  • Size

    100ug

  • Price

    $480

1
  • SKU

    YCP5427-1mg

  • Size

    1mg

  • Price

    $2,970

1