Human TNFRSF4 / OX40 (Leu29-Ala216) protein, with C-Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5699-100ug

  • Size

    100ug

  • Price

    $420

1
  • SKU

    YCP5699-1mg

  • Size

    1mg

  • Price

    $2,805

1
This recombinant protein contains Leu29-Ala216 of UniProt P43489. It has a human IgG1 Fc tag at the C-terminus. The protein's calculated molecular weight is 46.8 kDa, migrating at 50-67 kDa in reducing SDS-PAGE due to glycosylation.
gene name TNFRSF4
gene id UniProt P43489
host species Human
production host HEK293
tags C-Fc
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human OX40 protein

ELISA: Immobilized Human OX40 Ligand-His (active trimer) at 5 ug/mL (100 uL/well) binds to Human OX40-Fc with a linear range of 0.4-3 ng/mL.

Quantitative Analysis of CTLA-4 X OX40 Bispecific Antibody in Human Serum by Intact Assay. Human OX40-Fc at 2 ug/mL was coated on plate, increasing concentrations of CTLA-4 and OX40 Bispecific Antibody in 50% Human serum were added, then Biotinylated Human CTLA-4-Fc-Avi at 0.2 ug/mL was added. Detection was performed using HRP-conjugated streptavidin with sensitivity of 2 ng/mL .


PRODUCT

  • SKU

    YCP5699-100ug

  • Size

    100ug

  • Price

    $420

1
  • SKU

    YCP5699-1mg

  • Size

    1mg

  • Price

    $2,805

1