Human PDGFB / PDGF-BB (Ser82-Thr190) protein, with C-6xHis-Avi Tag, expressed from E. coli

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  • SKU

    YCP5760-50ug

  • Size

    50ug

  • Price

    $450

1
  • SKU

    YCP5760-200ug

  • Size

    200ug

  • Price

    $825

1
  • SKU

    YCP5760-1mg

  • Size

    1mg

  • Price

    $3,300

1
This recombinant protein contains Ser82-Thr190 of UniProt P01127. It has a His tag at the C-terminus, followed by an Avi tag. The protein's calculated molecular weight is 15.1 kDa, migrating at 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).
gene name PDGFB
host species Human
production host E. coli
tags C-6xHis-Avi
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 0.085% TFA in 30% ACN, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Unconjugated Human PDGF-BB protein

ELISA: Immobilized Unconjugated Human PDGF-BB-His-Avi at 1 ug/mL (100 uL/well) binds to Human PDGF R beta-Fc with a linear range of 0.5-16 ng/mL.


PRODUCT

  • SKU

    YCP5760-50ug

  • Size

    50ug

  • Price

    $450

1
  • SKU

    YCP5760-200ug

  • Size

    200ug

  • Price

    $825

1
  • SKU

    YCP5760-1mg

  • Size

    1mg

  • Price

    $3,300

1