Human VEGFA / VEGF165 (Ala27-Arg191) protein, with N-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5967-50ug

  • Size

    50ug

  • Price

    $493.5

1
  • SKU

    YCP5967-1mg

  • Size

    1mg

  • Price

    $4,440

1
This recombinant protein contains Ala27-Arg191 of UniProt P15692. It has a His tag at the N-terminus. The protein's calculated molecular weight is 21.1 kDa, migrating at 25-27 kDa in reducing SDS-PAGE due to glycosylation.
gene name VEGFA
host species Human
production host HEK293
tags N-10xHis
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human VEGF165 protein

ELISA: Immobilized Human VEGF R1&VEGF R2-Fc at 2 ug/mL (100 uL/well) binds to Human VEGF165-His with a linear range of 0.004-0.063 ug/mL.


PRODUCT

  • SKU

    YCP5967-50ug

  • Size

    50ug

  • Price

    $493.5

1
  • SKU

    YCP5967-1mg

  • Size

    1mg

  • Price

    $4,440

1