Human / Rhesus macaque CD40LG / CD40 Ligand (Met113-Leu261) protein, with N-mFc (mIgG2a) Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8175-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP8175-1mg

  • Size

    1mg

  • Price

    $3,270

1
This recombinant protein contains Met113-Leu261 of UniProt P29965. It has a mouse IgG2a Fc tag at the N-terminus. The protein's calculated molecular weight is 43.1 kDa, migrating at 45-50 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD40LG
gene id UniProt P29965
host species Human / Rhesus macaque
production host HEK293
tags N-mFc (mIgG2a)
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human / Rhesus macaque CD40 Ligand protein

ELISA: Immobilized Human CD40 Protein-His at 1 ug/mL (100 uL/well) binds to Human / Rhesus macaque CD40 Ligand Protein-mFc with a linear range of 0.1-4 ng/mL.

ELISA: Immobilized Cynomolgus CD40 Protein-His at 5 ug/mL (100 uL/well) binds to Human / Rhesus macaque CD40 Ligand Protein-mFc with a linear range of 0.3-5 ng/mL .


PRODUCT

  • SKU

    YCP8175-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP8175-1mg

  • Size

    1mg

  • Price

    $3,270

1