Human IL7R / IL-7 R alpha (Glu21-Gly236) protein, with C-mFc (mIgG2a) Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8372-100ug

  • Size

    100ug

  • Price

    $480

1
  • SKU

    YCP8372-1mg

  • Size

    1mg

  • Price

    $3,465

1
This recombinant protein contains Glu21-Gly236 of UniProt P16871 (I66T, V138I). It has a mouse IgG2a Fc tag at the C-terminus. The protein's calculated molecular weight is 52.0 kDa, migrating at 65-115 kDa in reducing SDS-PAGE due to glycosylation.
gene name IL7R
gene id UniProt P16871 (I66T, V138I)
host species Human
production host HEK293
tags C-mFc (mIgG2a)
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in Tris with Glycine, Arginine and NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human IL-7 R alpha protein

ELISA: Immobilized Human IL-7 Protein at 2 ug/mL (100 uL/well) binds to Human IL-7 R alpha-mFc with a linear range of 4-31 ng/mL.

ELISA: Biotinylated Human TSLP (R127A, R130A)-His-Avi immobilized at 2 ug/mL (100 uL/well) via precoated 5 ug/mL (100 uL/well) of Human TSLP R-Fc , binds to binds to Human IL-7 R alpha-mFc with a linear range of 1-20 ng/mL .


PRODUCT

  • SKU

    YCP8372-100ug

  • Size

    100ug

  • Price

    $480

1
  • SKU

    YCP8372-1mg

  • Size

    1mg

  • Price

    $3,465

1