Human NGF/pro-Beta-NGF protein, tag-free, expressed from E.coli

PRODUCT

This recombinant protein contains the amino acid sequence of Uniprot ID P01138. It is tag-free, with a molecular weight of 25 KDa.

gene name	NGF
gene id	Uniprot ID: P01138
alias	Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB, -NGF, pro-Beta NGF
host species	Human
production host	E.coli
tags	None
purity	Greater than 95% as determined by reducing SDS-PAGE.

endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation	Lyophilized from a 0.2 um filtered solution of 20mM Tris-HCl,500mM NaCl,5%Trehalose,5%Mannitol,0.01%tween80,1mM EDTA,pH8.0.
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions.
storage	Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.