Human NGF / Beta-NGF (Ser122-Ala241) protein, Tag-free, expressed from HEK293

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PRODUCT

  • SKU

    YCD1150-500ug

  • Size

    500ug

  • Price

    $1,170

1
This recombinant protein contains Ser122-Ala241 of UniProt P01138. It is tag-free. The protein's calculated molecular weight is 13.5 kDa, migrating at 14-15 kDa in reducing SDS-PAGE due to glycosylation.
gene name NGF
gene id UniProt P01138
host species Human
production host HEK293
tags Tag-free
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 20 mM NaAC, 150 mM NaCl, pH5.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human Beta-NGF protein

ELISA: Immobilized Human Beta-NGF Protein at 1 ug/mL (100 uL/well) binds to Human TrkA (33-417)-mFc with a linear range of 1-16 ng/mL.

ELISA: Immobilized Human Beta-NGF Protein at 1 ug/mL (100 uL/well) binds to Human TrkA (192-402)-mFc with a linear range of 1-16 ng/mL .


PRODUCT

  • SKU

    YCD1150-500ug

  • Size

    500ug

  • Price

    $1,170

1