Human SIRPA (Glu31-Tyr373) protein, with C-hFc and 6xHis Tag, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    DMP2095-50ug

  • Size

    50 ug

  • Price

    $560

1
  • SKU

    DMP2095-100ug

  • Size

    100 ug

  • Price

    $840

1
This recombinant protein contains Glu31-Tyr373 of Uniprot ID P78324. It has a C-hFc and 6xHis Tag, with a calculated molecular weight of 70-98 kDa.
gene name SIRPA
gene id Uniprot ID: P78324
alias SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS
host species Human
production host HEK293
tags C-hFc and 6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD47, mFc-His tagged protein binds to its native ligand Human SIRP&alpha;, hFc-His tagged protein in a linear range of 3.3-26.37 ng/ml.

A: Human SIRP&alpha;, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRP&alpha;, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRP&alpha;, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRP&alpha;, hFc-His tagged protein after five freeze-thaw cycles.

Human SIRP&alpha;, hFc-His Tag, SDS-PAGE analysis under reducing conditions.

Lane 1: Human SIRP&alpha;, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRP&alpha;, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRP&alpha;, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRP&alpha;, hFc-His tagged protein after five freeze-thaw cycles.


PRODUCT

  • SKU

    DMP2095-50ug

  • Size

    50 ug

  • Price

    $560

1
  • SKU

    DMP2095-100ug

  • Size

    100 ug

  • Price

    $840

1