Mouse SIRPA / SIRP alpha (Lys32-Asn373, G365D) protein, with C-mFc (mIgG2a) Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8535-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP8535-1mg

  • Size

    1mg

  • Price

    $3,270

1
This recombinant protein contains Lys32-Asn373 of UniProt P97797 (G365D). It has a mouse IgG2a Fc tag at the C-terminus. The protein's calculated molecular weight is 65.0 kDa, migrating at 90-130 kDa in reducing SDS-PAGE due to glycosylation.
gene name SIRPA
gene id UniProt P97797 (G365D)
host species Mouse
production host HEK293
tags C-mFc (mIgG2a)
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 100 mM Glycine, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Mouse SIRP alpha protein

ELISA: Immobilized Mouse SIRP alpha Protein-mFc at 2 ug/mL (100 uL/well) binds to Mouse CD47-Fc with a linear range of 8-63 ng/mL.

ELISA: Immobilized Human CD47 at 10 ug/mL (100 uL/well) binds to Mouse SIRP alpha Protein-mFc with a linear range of 0.02-0.313 ug/mL .


PRODUCT

  • SKU

    YCP8535-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP8535-1mg

  • Size

    1mg

  • Price

    $3,270

1