Home/ Products / Recombinant Rabbit McAbs
DMR1382-100ug
100 ug
$657
DMR1382-500ug
500 ug
$1,971
| target | TNFRSF17 |
| alias | TNFRSF17 |
| gene id | Uniprot ID: Q02223 |
| host species | Rabbit |
| production host | HEK293 |
| reactivity | Human |
| clonality | Monoclonal |
| clone number | DM4 |
| iso type | Rabbit IgG |
| formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| applications | ELISA, Flow Cytometry, IF, IP |
| dilution | Flow Cyt 1:100; IP 1:30 |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
A. Flow cytometry analysis of anti-BCMA (DM4) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
A. Flow cytometry analysis of anti-BCMA (DM4) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Western blot analysis of BCMA protein using anti-BCMA antibody at 1:1000 dilution. Secondary antibody: HRP Goat anti-Rabbit IgG (H L) at 1:5000 dilution. 1. K562 cell lysate (negative control); 2. K562 cell lysate with overexpressed human BCMA proteins; 3. MM.1S cell lysate (native BCMA protein)
Phylogenetic analysis of different anti-BCMA DimAb clones.A) heavy chain and B) Light chain.
Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4?. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
ELISA plate was coated with recombinant BCMA-hFc fusion protein , followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.
Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with FLAG tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-FLAG monoclonal antibody.
DMR1382-100ug
100 ug
$657
DMR1382-500ug
500 ug
$1,971