Biotinylated mouse Integrin alpha V beta 5 (ITGAV, ITGB5) protein, with C-10xHis-Avi Tag, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    YCD1577-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1577-200ug

  • Size

    200ug

  • Price

    $2,070

1
This recombinant protein contains Phe31-Val988 and Gly24-Asn719 of UniProt P43406. The biotinylated heterdimer protein has a calculated MW of 114.2 kDa (ITGAV) and 77.5 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a His tag and subunit ITGB5 contains no tag but a basic tail at the C-terminus.The protein migrates at 80-90 kDa (ITGB5) and 125-145 kDa (ITGAV) under non-reducing conditions in SDS-PAGE due to glycosylation.
gene name ITGAV, ITGB5
gene id UniProt P43406 and O70309
host species Mouse
production host HEK293
tags C-10xHis-Avi and Tag-free
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 150 mM NaCl,pH 7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Biotinylated Mouse ITGAV&ITGB5 Heterodimer protein

ELISA: Immobilized Human Fibronectin at 50 ug/mL (100 uL/well) binds to Biotinylated Mouse ITGAV&ITGB5 Heterodimer Protein-His-Avi&Tag Free with a linear range of 0.625-10 ug/mL .


PRODUCT

  • SKU

    YCD1577-25ug

  • Size

    25ug

  • Price

    $525

1
  • SKU

    YCD1577-200ug

  • Size

    200ug

  • Price

    $2,070

1