Human Integrin alpha 4 beta 7 (ITGA4 and ITGB7) protein, with C-6xHis, expressed from HEK293

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PRODUCT

  • SKU

    YCD1809-100ug

  • Size

    100ug

  • Price

    $615

1
  • SKU

    YCD1809-1mg

  • Size

    1mg

  • Price

    $4,755

1
This recombinant protein contains Tyr34-Arg620 and Glu20-Pro521 of UniProt P13612, P26010. The heterodimer has a calculated MW of 59.5 kDa (ITGA4) and 59.4 kDa (ITGB7), migrating at 75-95 kDa in reducing SDS-PAGE due to glycosylation.
gene name ITGA4, ITGB7
gene id UniProt P13612, P26010
host species Human
production host HEK293
tags C-6xHis & Tag-free
purity > 90% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 150 mM NaCl, pH8.0, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human ITGA4&ITGB7 Heterodimer protein

ELISA: Immobilized Human ITGA4&ITGB7 Heterodimer Protein, His Immobilized Human ITGA4&ITGB7 Heterodimer Protein-His&Tag Free at 2 ug/mL (100 uL/well) binds to Human MAdCAM-1-Fc with a linear range of 0.05-0.8 ng/mL.

ELISA: Immobilized Human ITGA4&ITGB7 Heterodimer Protein-His&Tag Free at 5 ug/mL (100 uL/well) binds to Biotinylated Human VCAM-1-Fc-Avi with a linear range of 0.4-6 ng/mL .


PRODUCT

  • SKU

    YCD1809-100ug

  • Size

    100ug

  • Price

    $615

1
  • SKU

    YCD1809-1mg

  • Size

    1mg

  • Price

    $4,755

1