Human CD274 / PD-L1 (Phe19-Arg238) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP1997-100ug

  • Size

    100ug

  • Price

    $495

1
  • SKU

    YCP1997-1mg

  • Size

    1mg

  • Price

    $3,630

1
This recombinant protein contains Phe19-Arg238 of UniProt Q9NZQ7. It has a His tag at the C-terminus. The protein's calculated molecular weight is 26.0 kDa, migrating at 30-35 kDa in reducing SDS-PAGE due to glycosylation.
gene name CD274
gene id UniProt Q9NZQ7
host species Human
production host HEK293
tags C-6xHis
purity > 98% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Human PD-L1 protein

ELISA: Immobilized Human PD-L1-His at 1 ug/mL (100 uL/well) binds to Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL.

ELISA: Immobilized Human PD-L1-His at series of concentration on MonoRab?? Anti-His Tag (C-term) Antibody precoated (0.1 ug/well) plate, binds to Human PD-1-mFc at 5 ug/mL (100 uL/well) with a linear range of 10-78 ng/mL .


PRODUCT

  • SKU

    YCP1997-100ug

  • Size

    100ug

  • Price

    $495

1
  • SKU

    YCP1997-1mg

  • Size

    1mg

  • Price

    $3,630

1