Mouse PCSK9 (Gln35-Gln694) protein, with C-6xHis Tag, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    YCP5717-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP5717-1mg

  • Size

    1mg

  • Price

    $3,270

1
This recombinant protein contains Gln35-Gln694 of UniProt Q80W65. It has a His tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non?covalent interactions and with a calculated MW of 13.9 kDa and 58.2 kDa respectively, migrating at 17 kDa and 65 kDa in reducing SDS-PAGE due to glycosylation.
gene name PCSK9
gene id UniProt Q80W65
host species Mouse
production host HEK293
tags C-6xHis
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in PBS, pH7.4, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Mouse PCSK9 protein

ELISA: Immobilized Human LDL R-Fc at 5 ug/mL (100 uL/well) binds to Mouse PCSK9-His with a linear range of 0.078-0.313 ug/mL.

ELISA: Immobilized Mouse PCSK9-His at 1 ug/mL (100 uL/well) on Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) precoated (0.1 ug/well) plate binds to Evolocumab with a linear range of 2-31 ng/mL .


PRODUCT

  • SKU

    YCP5717-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP5717-1mg

  • Size

    1mg

  • Price

    $3,270

1