Rat PCSK9 (Gln31-Gln691) protein, with C-10xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5719-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5719-1mg

  • Size

    1mg

  • Price

    $3,270

1
This recombinant protein contains Gln31-Gln691 of UniProt P59996. It has a His tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non?covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively, migrating at 16 kDa and 60-66 kDa in reducing SDS-PAGE due to glycosylation.
gene name PCSK9
gene id UniProt P59996
host species Rat
production host HEK293
tags C-10xHis
purity > 95% as determined by SDS-PAGE
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein was lyophilized from a solution in 50 mM Tris, 150 mM NaCl, pH7.5, with trehalose added as protectant.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of Rat PCSK9 protein

ELISA: Immobilized Human LDL R-Fc at 5 ug/mL (100 uL/well) binds to Rat PCSK9-His with a linear range of 0.156-0.625 ug/mL.


PRODUCT

  • SKU

    YCP5719-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5719-1mg

  • Size

    1mg

  • Price

    $3,270

1