Human TNF/TNFA (Val77-Leu233) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP2159-50ug

  • Size

    50 ug

  • Price

    $609

1
  • SKU

    DMP2159-100ug

  • Size

    100 ug

  • Price

    $914

1
This recombinant protein contains Val77-Leu233 of Uniprot ID P01375. It has a C-6xHis Tag, with a calculated molecular weight of 18.1 kDa.
gene name TNF
gene id Uniprot ID: P01375
alias DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
host species Human
production host HEK293
tags C-6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 1 ug/ml (100 ul/well) Human TNFa , His tagged protein binds to anti-TNFa neutralizing antibody ([getskuurl sku"in a linear range of 0.64-16 ng/ml.

Human TNF&alpha; Protein, His Tag, SDS-PAGE analysis under reducing conditions.

ELISA plate coated with 2 ug/mL (100 uL/well) Human TNF&alpha; Protein, His Tag binds to anti-TNFa (Certolizumab biosimilar) mAb in a linear range of 0.64?16 ng/mL.

ELISA plate coated with 2 ug/mL (100 uL/well) Human TNF&alpha; Protein, His Tag binds to anti-TNFa (golimumab biosimilar) mAb in a linear range of 3.20?80 ng/mL.


PRODUCT

  • SKU

    DMP2159-50ug

  • Size

    50 ug

  • Price

    $609

1
  • SKU

    DMP2159-100ug

  • Size

    100 ug

  • Price

    $914

1