Human TNF protein, with C-6xHis Tag, expressed from E.coli

PRODUCT

This recombinant protein contains the amino acid sequence of Uniprot ID P01375. It has a C-6xHis Tag, with a molecular weight of 21.8 KDa.

gene name	TNF
gene id	Uniprot ID: P01375
alias	Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2
host species	Human
production host	E.coli
tags	C-6xHis Tag
purity	Greater than 95% as determined by reducing SDS-PAGE.

endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation	Lyophilized from a 0.2 um filtered solution of 20mM PB, 100mM NaCl, pH 8.0.
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions.
storage	Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.