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DMS1164-100ug
100 ug
$350
target | TNF |
alias | DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα |
gene id | Uniprote ID: P01375 |
host species | Human |
reactivity | Human |
iso type | IgG1 |
formulation | Lyophilized from sterile PBS, pH 7.4. Typically, 5% - 8% trehalose is added as a protective agent prior to lyophilization. |
reconstitution | Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 minutes with occasional gentle tapping. It is recommended that 0.1% HSA be added as a carrier protein for long-term storage. |
applications | ELISA; Flow Cytometry |
dilution | ELISA 1:5000-10000; Flow Cyt 1:100 |
shipping | Shipped at ambient conditions. |
storage | Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months. |
note | Other commonly used names in publications refering to this antibody: D2E7 |
ELISA plate coated with 1 ug/ml (100 ul/well) Human TNFa , His tagged protein binds to anti-TNFa neutralizing antibody in a linear range of 0.3-500 ng/ml.
Flow cytometry analysis of anti-TNFa mAb 1 ug/ml (Adalimumab) on Expi293 cells transfected with human TNFa (Blue histogram)or Expi293 transfected with irrelevant protein (Red histogram) .
DMS1164-100ug
100 ug
$350